Publications

A Two-Antibody Pan-Ebolavirus Cocktail Confers Broad Therapeutic Protection in Ferrets and Nonhuman Primates

Zachary A. Bornholdt, Andrew S. Herbert, Chad E. Mire, Shihua He, Robert W. Cross, Anna Z. Wec, Dafna M. Abelson, Joan B. Geisbert, Rebekah M. James, Md Niaz Rahim, Wenjun Zhu, Viktoriya Borisevich, Logan Banadyga, Bronwyn M. Gunn, Krystle N. Agans, Ariel S. Wirchnianski, Eileen Goodwin, Kevin Tierney, William S. Shestowsky, Ognian Bohorov, Natasha Bohorova, Jesus Velasco, Eric Ailor, Do Kim, Michael H. Pauly, Kevin J. Whaley, Galit Alter, Laura M. Walker, Kartik Chandran, Larry Zeitlin, Xiangguo Qiu, Thomas W. Geisbert, John M. Dye

Development of a Human Antibody Cocktail that Deploys Multiple Functions to Confer Pan-Ebolavirus Protection

Anna Z. Wec, Zachary A. Bornholdt, Shihua He, Andrew S. Herbert, Eileen Goodwin, Ariel S. Wirchnianski, Bronwyn M. Gunn, Zirui Zhang, Wenjun Zhu, Guodong Liu, Dafna M. Abelson, Crystal L. Moyer, Rohit K. Jangra, Rebekah M. James, Russell R. Bakken, Natasha Bohorova, Ognian Bohorov, Do H. Kim, Michael H. Pauly, Jesus Velasco, Robert H. Bortz III, Kevin J. Whaley, Tracey Goldstein, Simon J. Anthony, Galit Alter, Laura M. Walker, John M. Dye, Larry Zeitlin, Xiangguo Qiu, Kartik Chandran

Systematic comparison of respiratory syncytial virus-induced memory B cell responses in two anatomical compartments

Laila Shehata, Wendy F. Wieland-Alter, Daniel P. Maurer, Eunice Chen, Ruth I. Connor, Peter F. Wright, Laura M. Walker

Structural basis of broad ebolavirus neutralization by a human survivor antibody

Brandyn R. West, Anna Z. Wec, Crystal L. Moyer, Marnie L. Fusco, Philipp A. Ilinykh, Kai Huang, Ariel S. Wirchnianski, Rebekah M. James, Andrew S. Herbert, Sean Hui, Eileen Goodwin, Katie A. Howell, Shweta Kailasan, M. Javad Aman, Laura M. Walker, John M. Dye, Alexander Bukreyev, Kartik Chandran, Erica Ollmann Saphire

Deamidation and isomerization liability analysis of 131 clinical-stage antibodies

Xiaojun Lu, R. Paul Nobrega, Heather Lynaugh, Tushar Jain, Kyle Barlow, Todd Boland, Arvind Sivasubramanian, Maximiliano Vásquez, Yingda Xu

Structure, heterogeneity and developability assessment of therapeutic antibodies

Yingda Xu, Dongdong Wang, Bruce Mason, Tony Rossomando, Ning Li, Dingjiang Liu, Jason K. Cheung, Wei Xu, Smita Raghava, Amit Katiyar, Christine Nowak, Tao Xiang, Diane D. Dong, Joanne Sun, Alain Beck, Hongcheng Liu

Passive immunotherapy of viral infections: 'super-antibodies' enter the fray

Laura M. Walker, Dennis R. Burton

Infants Infected with Respiratory Syncytial Virus Generate Potent Neutralizing Antibodies that Lack Somatic Hypermutation

Eileen Goodwin, Morgan S.A. Gilman, Daniel Wrapp, Man Chen, Joan O. Ngwuta, Syed M. Moin, Patricia Bai, Arvind Sivasubramanian, Ruth I. Connor, Peter F. Wright, Barney S. Graham, Jason S. McLellan, Laura M. Walker

Zika virus activates de novo and cross-reactive memory B cell responses in dengue-experienced donors

Thomas F. Rogers, Eileen Goodwin, Bryan Briney, Devin Sok, Nathan Beutler, Alexander Strubel, Rebecca Nedellac, Khoa Le, Michael E. Brown, Dennis R. Burton, Laura M. Walker

Chaperone proteins as single component reagents to assess antibody nonspecificity

Ryan Kelly, James Geoghegan, Jared Feldman, Tushar Jain, Monique Kauke, Jasmine Le, Jessie Zhao, K. Dane Wittrup

Rapid assessment of oxidation via middle-down LCMS correlates with methionine side-chain solvent-accessible surface area for 121 clinical stage monoclonal antibodies

Rong Yang, Tushar Jain, Heather Lynaugh, R. Paul Nobrega, Xiaojun Lu, Todd Boland, Irina Burnina, Tingwan Sun, Isabelle Caffry, Michael Brown, Xiaoyong Zhu, Asparouh Lilov, Yingda Xu

Biophysical properties of the clinical-stage antibody landscape

Tushar Jain, Tingwan Sun, Stéphanie Durand, Amy Hall, Nga Rewa Houston, Juergen H. Nett, Beth Sharkey, Beata Bobrowicz, Isabelle Caffry, Yao Yu, Yuan Cao, Heather Lynaugh, Michael Brown, Hemanta Baruah, Laura T. Gray, Eric M. Krauland, Yingda Xu, Maximiliano Vásquez, K. Dane Wittrup

Database-Centric Method for Automated High-Throughput Deconvolution and Analysis of Kinetic Antibody Screening Data

R. Paul Nobrega, Michael Brown, Cody Williams, Chris Sumner, Patricia Estep, Isabelle Caffry, Yao Yu, Heather Lynaugh, Irina Burnina, Asparouh Lilov, Jordan Desroches, John Bukowski, Tingwan Sun, Jonathan P. Belk, Kirt Johnson, Yingda Xu

Antibodies from a Human Survivor Define Sites of Vulnerability for Broad Protection against Ebolaviruses

Anna Z. Wec, Andrew S. Herbert, Charles D. Murin, Elisabeth K. Nyakatura, Dafna M. Abelson, J. Maximilian Fels, Shihua He, Rebekah M. James, Marc-Antoine de La Vega, Wenjun Zhu, Russell R. Bakken, Eileen Goodwin, Hannah L. Turner, Rohit K. Jangra, Larry Zeitlin, Xiangguo Qiu, Jonathan R. Lai, Laura M. Walker, Andrew B. Ward, John M. Dye, Kartik Chandran, Zachary A. Bornholdt

Prediction of delayed retention of antibodies in hydrophobic interaction chromatography from sequence using machine learning

Tushar Jain, Todd Boland, Asparouh Lilov, Irina Burnina, Michael Brown, Yingda Xu, Maximiliano Vásquez

Broad epitope coverage of a human in vitro antibody library

Arvind Sivasubramanian, Patricia Estep, Heather Lynaugh, Yao Yu, Adam Miles, Josh Eckman, Kevin Schutz, Crystal Piffath, Nadthakarn Boland, Rebecca Hurley Niles, Stéphanie Durand, Todd Boland, Maximiliano Vásquez, Yingda Xu, Yasmina Abdiche

Rapid profiling of RSV antibody repertoires from the memory B cells of naturally infected adult donors

Morgan S. A. Gilman, Carlos Castellanos, Man Chen, Joan O. Ngwuta, Eileen Goodwin, Syed M. Moin, Vicente Mas, Jose A. Melero, Peter F. Wright, Barney S. Graham, Jason S. McLellan, Laura M. Walker

Purification of common light chain IgG-like bispecific antibodies using highly linear pH gradients

Beth Sharkey, Sarat Pudi, Ian Wallace Moyer, Lihui Zhong, Hemanta Baruah, Heather Lynaugh, Sampath Kumar, K. Dane Wittrup, Juergen H. Nett

High throughput cross-interaction measures for human IgG1 antibodies correlate with clearance rates in mice

Ryan L Kelly, Tingwan Sun, Tushar Jain, Isabelle Caffry, Yao Yu, Yuan Cao, Heather Lynaugh, Michael Brown, Maximiliano Vásquez, K Dane Wittrup, Yingda Xu

Context matters: the importance of dimerization-induced conformation on Staphylococcus aureus for the generation of neutralizing antibodies

Adriana Badarau, Harald Rouha, Stefan Malafa, Michael B. Battles, Laura Walker, Nels Nielson, Ivana Dolezilkova, Astrid Teubenbacher, Srijib Banerjee, Barbara Maierhofer, Susanne Weber, Lukas Stulik, Derek T. Logan, Martin Welin, Irina Mirkina, Clara Pleban, Gerhild Zauner, Karen Gross, Michaela Jagerhofer, Zoltan Magyarics, Eszter Nagy

Target-independent variable region mediated effects on antibody clearance can be FcRn independent

Ryan L. Kelly, Yao Yu, Tingwan Sun, Isabelle Caffry, Heather Lynaugh, Michael Brown, Tushar Jain, Yingda Xu, K. Dane Wittrup

Isolation of potent neutralizing antibodies from a survivor of the 2014 Ebola virus outbreak

Zachary A. Bornholdt, Hannah L. Turner, Charles D. Murin, Wen Li, Devin Sok, Colby A. Souders, Ashley E. Piper, Arthur Goff, Joshua D. Shamblin, Suzanne E. Wollen, Thomas R. Sprague, Marnie L. Fusco, Kathleen B. J. Pommert, Lisa A. Cavacini, Heidi L. Smith, Mark Klempner, Keith A. Reimann, Eric Krauland, Tillman U. Gerngross, K. Dane Wittrup, Erica Ollmann Saphire, Dennis R. Burton, Pamela J. Glass, Andrew B. Ward, Laura M. Walker

Understanding ForteBio's Sensors for High-Throughput Kinetic and Epitope Screening for Purified Antibodies and Yeast Culture Supernatant

Yao Yu, Scott Mitchell, Heather Lynaugh, Michael Brown, R. Paul Nobrega, Xiaoyong Zhi, Tingwan Sun, Isabelle Caffry, Yuan Cao, Rong Yang, Irina Burnina, Yingda Xu, Patricia Estep

Five birds, one stone: Neutralization of a-hemolysin and 4 bi-component leukocidins of Staphylococcus aureus with a single human monoclonal antibody

Harald Rouha, Adriana Badarau, Zehra C. Visram, Michael B. Battles, Bianka Prinz, Zoltan Magyarics, Gabor Nagy, Irina Mirkina, Lukas Stulik, Manuel Zerbs, Michaela Jagerhofer, Barbara Maierhofer, Astrid Teubenbacher, Ivana Dolezilkova, Karin Gross, Srijib Banerjee, Gehild Zauner, Stefan Malafa, Jakub Zmajkovic, Sabine Maier, Robert Mabry, Eric Krauland, K. Dane Wittrup, Tillman U. Gerngross, Eszter Nagy

High-Throughput Screening for Developability During Early-Stage Antibody Discovery Using Self-Interaction Nanoparticle Spectroscopy

Yuqi Liu, Isabelle Caffry, Jiemin Wu, Steven B. Geng, Tushar Jain, Tingwan Sun, Felicia Reid, Yuan Cao, Patricia Estep, Yao Yu, Maximiliano Vásquez, Peter M. Tessier, Yingda Xu

High Throughput Detection of Antibody Self-Interaction by Bio-Layer Interferometry

Tingwan Sun, Felicia Reid, Yuqi Liu, Yuan Cao, Patricia Estep, Claire Nauman, Yingda Xu

Addressing polyspecificity of antibodies selected from an in vitro yeast presentation system: a FACS-based, high-throughput selection and analytical tool

Yingda Xu, William Roach, Tingwan Sun, Tushar Jain, Bianka Prinz, Ta-Yi Yu, Joshua Torrey, Jerry Thomas, Piotr Bobrowicz, Maximiliano Vásquez, K. Dane Wittrup, Eric Krauland

High Throughput Solution-Based Measurement of Antibody-Antigen Affinity and Epitope Binning

Patricia Estep, Felicia Reid, Claire Nauman, Yuqi Liu, Tingwan Sun, Joanne Sun, Yingda Xu